CellTank integrating centrifugal pump

CP-SUB with 150 ml scaffold regulating biomass with temperature

CellTank-34 is a novel Continuous-Perfusion-Single-Use-Bioreactor (CP-SUB) designed for steady-state continuous cultivation at high density of adherent as well as suspension type of mammalian cells for expression of biologics. CellTank-34 CP-SUB is a ready-to-use, pre-assembled in a pre-sterilized package designed for biomass control by temperature regulation.

CellTank-34 is designed for Magnetic-Stirrer-Table (MST) drive operation handled by most Process-Control-Systems (PCS). So with your in-house PCS you are able to operate the CellTank-34 at max cell density and highest possible output.

CellTank-34 general specification:

  • 150 ml PET scaffold, matrix either for suspension CHO or adherent CHO cells 
  • Polyamide cover integrated 100% fluid tight with vessel
  • PC (PolyCarbonate) vessel OD 137 mm
  • Polyamide reactor body integrating envelopes filled with PET fibre matrix for enhanced bio-compatibility cultivating either adherent or suspension cell lines
  • 4-pole permanent magnet operated polyamide high performance centrifugal pump facilitating simple operation on clock-wise rotation
  • Drive by any PCS with >40 watt servo motor via Magnetic-Stirrer-Table (MST)
  • A - Thermal control by Heating-Support-Foot (pn# 2266-E) work on both 115 and 230 VAC
  • B - Thermal control by traditional Electrical-Heating-Blanket (75 x 350 mm) (pn# 22370 or 22365)
  • Efficient elimination of bubble to cell contact and kLa values above 40 sparging with air
  • Very efficient head space to liquid surface contact for high efficient CO2 control
  • Delivered high precision E-beam irradiated in dual polyester foil bags

CellTank model, p/n

34-0150

Vessel Volume/size, ml

2,500

Media reservoir volume, ml

1,200

Working Volume matrix, ml

150

Matrix surface area (for adherent CHO) cm2

36x10E+03

Required MST operation range, rpm

250 - 650

Required power delivered by MST, watt

>25

Vessel OD, mm

137

Weight no media, grams

1.200

CellTank facilitates the use of PG 13,5 x 120 mm classical format Single-Use-Sensor's (SUS):

  1. One pre-installed classical signal OneFerm pH SUS
  2. One pre-installed non-invasive VisiWell for dO2 Re-Usable-Sensor from Hamilton (VisiFerm not included)
  3. One port pre-installed with capacitance bio-mass SUS – PICO from Aber (alternatively your own 12 mm capacitance Re-Usable-Sensor Hamilton installed under the hood)
  4. Extra port for optional pre-installed Glucose SUS 

All CellTank further integrate the following Single-Use-Sensor's (SUS):

  • Non-invasive temperature well for <ID 6,5 mm sensor – facilitate your PCS standard probe for on-line measurements
  • Integrated media re-circulation mass flow rotameter – manual read-out of 0,4 - 2,4 litre/min
  • Scaffold pressure drop – manual read-out of 0 – 600 mm WC - alternatively PendoTech SUS
  • Media level with insulated SS electrodes in 1.200 ml and 1.500 ml height 

CellTank-34 available since 2013.

  • Check out more information under Support
  • Motor-Drive-System's for the Magnetic-Stirrer-Table (MST) available from Cronus-PCS