CellTank integrating diaphragm pump

CP-SUB with 150 ml scaffold regulating biomass via sequential bleeding

CellTank-35 is a novel Continuous-Processing-Single-Use-Bioreactor (CP-SUB) designed for steady state continuous cultivation of high density adherent as well as suspension type of mammalian cells for expression or proliferation of biologics. CellTank is ready-to-use, pre-assembled and facilitates integrated Single-Use-Sensor's and Single-Use-Pump all in a pre-sterilized and complete package. Designed for continues expression of product or proliferation of biomass by sequential product harvest or cell harvest performed by the integrated diaphragm pump. 

CellTank-35 is in the design process integrating one build-in Melpomene Air-Operated-Diaphragm-Positive-Displacement (AODPD) driven by the Eirene Drive Unit. CellTank general cultivation parameters controlled by a suitable standard Process-Control-System (PCS).

CellTank-35 general specification:

  • PET scaffold, matrix harbouring either suspension or adherent mammalian cells 
  • Polyamide cover integrated 100% fluid tight with vessel
  • Polyamide reactor body integrating two envelopes filled with polyester fibre matrix for enhanced bio-compatibility cultivating either adherent or suspension cells
  • Build in high performance diaphragm pump facilitating advanced operation such as; innoculation, media re-circulation, cell bleeding, harvest function
  • Efficient elimination of bubble to cell contact and kLa values above 40 sparging with air
  • Efficient head space to liquid surface contact for efficient CO2 control
  • Delivered high precision E-beam irradiated in dual polyester foil bags

CellTank model, p/n

35-0150

Vessel volume/size, ml

2,500

Media reservoir volume, ml

1,500

Working matrix volume, ml

150

Matrix surface area, CHO, cm2

36x10E+03

Vessel OD, mm

137

Weight, grams 1.500

CellTank-35 facilitate one Melpomene Air-Operated-Diaphragm-Positive-Displacement (AODPD) Single-Use-Pump (SUP):

  1. One integrated Melpomene SUP for cell inoculation, cell distribution inside the scaffold, harvest of product, harvest of cells for biomass control, harvest of cells as product

CellTank-35 facilitates the use of PG 13,5 x 120 mm classical format Single-Use-Sensor's (SUS):

  1. One pre-installed classical signal OneFerm pH sensor from Hamilton - included
  2. One pre-installed non-invasive VisiWell for dO2 Re-Usable-Sensor from Hamilton (VisiFerm not included)
  3. One port pre-installed with bio-mass sensor – PICO from Aber - included
  4. Extra port for optional pre-installed Glucose sensor 

CellTank-35 further integrate sensor's for measuring:

  • Temperature via PCS sensor in non-invasive well
  • Internal media re-circulation mass flow rotameter – SUS – manual read-out of 0,4 - 2,4 litre/min
  • Matrix pressure drop – SUS – 0 – 1,000 mm WC
  • Media level with an insulated SS electrode in 1.200 ml and 1.500 ml height

CellTank-35 is ready 2019 for development projects.